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Figure 3. Hipk1 regulates Wnt/β-catenin targets in the early embryo.(A) Morpholinos against Hipk1 expand expression domains of the Wnt-responsive dorsal patterning genes siamois (a–c) and Xnr3 (d–f). Embryos were injected with CoMO (40 ng per embryo), Hipk1MO1 (40 ng per embryo), or Hipk1MO2 (20 ng per embryo) in the DMZ of 2 dorsal blastomeres at the 4-cell stage. (B) Quantification of results in A. (C, D) ShRNA plasmids targeted against HIPK1 reduce HIPK1 levels in human embryonic kidney cells (C) and potentiate the response of a β-catenin reporter construct to co-transfected Wnt1 (D). (E) Morpholinos against Hipk1 eliminate or reduce expression of the Wnt-responsive genes MyoD (a–c) Xbra (d–f), and otx2 (g–i), but do not similarly eliminate expression of Xnot (j–l), a marker for axial mesoderm. Embryos were injected as in A. (F) Quantification of results in E. |
