| |
FIGURE 7.
Defective convergent extension movement induced by knockdown of Dhrs3. Expression of mesoderm and notochord marker genes brachyury (bra), not, and goosecoid (gsc), and CE marker gene papc in control embryos (A-D), in response to dhrs3 knockdown (E-H), dhrs3 overexpression (I-L), or exogenous atRA (M-P). The expression domain of bra and not was shortened by Dhrs3 knockdown (E and F) and embryos treated with atRA (M and N). A similar but less severe shortening of bra expression domain was observed in embryos overexpressing dhrs3 (I). The not expression domain remained in normal shape, but the signal was less intensified in embryos overexpressing dhrs3 (J). The expression of gsc was suppressed by Dhrs3 knockdown (G) and atRA treatment (O) but was unaffected by dhrs3 overexpression (K). papc expression domain was more diffused in Dhrs3 morphants (H) and atRA treated embryos (P) when compared with control embryos (D). Percentages of embryos showing the phenotypes were marked in the figure. All embryos are dorsal view. |
