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Experiment details for not

Tail formation as a continuation of gastrulation: the multiple cell populations of the Xenopus tailbud derive from the late ...

Tail formation as a continuation of gastrulation: the multiple cell populations of the Xenopus tailbud derive from the late blastopore lip.

Gene Clone Species Stages Anatomy
not.L laevis NF stage 10.5 mesoderm , dorsal marginal zone , upper blastopore lip
not.L laevis NF stage 11 dorsal marginal zone , axial mesoderm
not.L laevis NF stage 22 tail bud
not.L laevis NF stage 35 and 36 tail tip

  Fig. 1. Xnot2 marks the tip of the tail and its expression can be followed back through development to the dorsal lip. Embryos of various stages were hybridized with antisense Xnot2 RNA probes. Whole-mount hybridization was visualized with an alkaline phosphatase reaction. (A) Stage 35 (48 h) embryo, staining at the tip of the tail. (B) Stage 22 (24 h) embryo, staining at the tailbud. (C) Stage 11 (midgastrula,12 h), staining in prospective notochord cells. (D) Stage 10 (early gastrula, 10 h), staining on the dorsal marginal zone prior to convergence and extension movements. Arrow indicates the dorsal lip.