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Fig. 1. Xnot2 marks the tip of the tail and its expression can be followed back through development to the dorsal lip. Embryos of various stages were hybridized with antisense Xnot2 RNA probes. Whole-mount hybridization was visualized with an alkaline phosphatase reaction. (A) Stage 35 (48 h) embryo, staining at the tip of the tail. (B) Stage 22 (24 h) embryo, staining at the tailbud. (C) Stage 11 (midgastrula,12 h), staining in prospective notochord cells. (D) Stage 10 (early gastrula, 10 h), staining on the dorsal marginal zone prior to convergence and extension movements. Arrow indicates the dorsal lip. |