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Fig. 2.
Both NADPH–diaphorase and immunohistochemistry reveal NOS in neurons in tadpole optic tectum. Dorsal isup, and lateral is to the right.A, Several NADPH–diaphorase-positive neurons in the densely packed layer 6, the main retinorecipient cell layer, can be seen sending apical dendrites dorsally that penetrate the layer 9 neuropil. In addition, three neurons in layer 4 and a single neuron in layer 8 are labeled. Blood vessels (bv) are also labeled in this tissue. P indicates the pial membrane layer.B, A coronal section from another tectum was immunostained with a commercial polyclonal type I NOS antibody. Layers 6 and 7 are shown. Several neurons with a morphology similar to those in NADPH—diaphorase-stained tissue can be seen. Scale bar: A, 80 μm; B, 50 μm. |
