|
Gene |
Clone |
Species |
Stages |
Anatomy |
nono.L
|
|
laevis
|
NF stage 3 (4-cell)
to
NF stage 6.5
|
animal cap
|
nono.L
|
|
laevis
|
NF stage 11
|
ectoderm
,
neuroectoderm
,
neuroectoderm inner layer
,
dorsal
|
nono.L
|
|
laevis
|
NF stage 14
|
ectoderm
,
neuroectoderm
,
pre-chordal neural plate
,
chordal neural plate
,
neural plate
,
[+]
|
nono.L
|
|
laevis
|
NF stage 21
|
neuroectoderm
,
pre-chordal neural plate
,
chordal neural plate
,
optic vesicle
,
anterior neural fold
,
[+]
|
|
|
Fig. 5. Spatial distribution of Xp54nrb mRNA during Xenopus early development. Whole mount in situ hybridization is performed on embryos from stage 3 to stage 21. Photomicrographs of whole-mounts and sec- tions through the corresponding whole mounts for stage 3 (A), stage 6.5 (B), stage 11 (C,D), stage 14 (E,F) and stage 21 (G,H) are shown. (A,B) Xp54nrb transcript is detected at the animal pole of cleaving embryos (animal views). (C) Xp54nrb expression in mid-gastrula is found in the ectoderm and mesoderm with a strong expression in dorsal side (lateral view, dorsal on the left). This pattern is confirmed by section analysis (D) which showed labelled of the ectoderm (ec) and in the involuting meso- derm (mes). (E) At early neural stage, the expression is restricted to the developing nervous system (anterior view, dorsal side is up) with a strong expression underlying the neural plate. (F) Transverse section analysis of the stage 14 shows that Xp54nrb is expressed in the sensorial layer of the neuroectoderm (Nes) and absent in the epithelial layer of the neuroecto- derm (Nep), in the notochord (n) and in the somitic mesoderm (ms). (G) At neurula (stage 21, anterior view, dorsal side is up) Xp54nrb expression is high in the anterior neural territories; optic field (of), mesencephalon (Me) and rhombencephalon (Rh) are labelled. Transverse section analysis (H) in a posterior position shows that only the neural tube (nt) is stained at this level. Abbreviations: bl; blastoporal lip, d; dorsal, Ec; ectoderm, end;endoderm, mes; mesoderm, Me; mesencephalon n; notochord, Nes; sensorial layer of the neuroectoderm, Nep; epithelial layer of the neuroectoderm, of; optic field, nt; neural tube, Rh; rhombencephalon, sm; somitic mesoderm, v; ventral. Scale bars 0.5 mm in (A,B), 0.3 mm in (C,D,E,G,H) and 0.1 mm in (F). Black bars in (E,G) represent the position of the corresponding sections in (F,H) respectively. |
|
Gene |
Clone |
Species |
Stages |
Anatomy |
nono.L
|
|
laevis
|
NF stage 23
|
retina
,
brain
,
forebrain
,
midbrain
,
hindbrain
,
[+]
|
nono.L
|
|
laevis
|
NF stage 35 and 36
|
retina
,
brain
,
forebrain
,
midbrain
,
hindbrain
,
[+]
ventricular zone
,
mandibular crest
,
hyoid crest
,
branchial crest
,
ciliary marginal zone
,
branchial arch
,
eye
,
mouth primordium
,
lateral line placode
,
cranial neural crest
,
retinal ganglion cell layer
,
rhombomere
,
rhombomere R2
,
rhombomere R3
,
rhombomere R4
,
rhombomere R5
,
rhombomere R6
|
|
|
Fig. 6. Spatial distribution of Xp54nrb mRNA during Xenopus early organogenesis. Photomicrographs of whole-mounts and sections through the corresponding whole mounts for stage 23 (A, B) and stage 35 (C-F) embryos are shown. (A) Whole mount and section analysis (B) at stage 23 show Xp54nrb expressed in optic cup (oc), otic vesicle (ov), branchial arches (ba) and mesencephalon (Me) (whole mount, lateral view, anterior is left). (C) Anterior view at stage 35 shows similar labelling patterns to the stage 23 embryo with neuroretina, encephalon and spinal chord labelled. (D) In the transverse section at the trunk level, only the ventricular zone of the spinal cord is stained (white arrowheads). (E) Section at the head level through the eye (F), enlargement of E) the dorsal part of D the spinal chord, and the inner layer of the neuroretina are labelled.
No staining is detected in the lens or in the ciliary marginal zone (CMZ, black arrowhead in F). Abbreviations: ba; branchial arches, cg; cement gland, CMZ, ciliary marginal zone, G, ganglion cell layer, I, inner nuclear cell layer, L, lens, Me; mesencephalon, O, outer nuclear cell layer, ov; otic vesicle, oc; optic cup, Rh; rhombencephalon, RPE retinal pigmented epithelium, Te; telencephalon, Scale bars 0.5 mm (in A-D), and 0.1 mm (in E, F). |