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Fig. 4. Selective repression of organizer genes by dorsally injected δNXMyD88 RNA. (A–E) Normal expression in control embryos; (F–K) representative δNXMyD88-injected (300 pg) embryos, coinjected with 100 pg nuclear β-galactosidase RNA as lineage tracer; numbers to the right indicate phenotypic penetrance. (L) Quantitative RT-PCR analysis for direct target genes of maternal β-catenin; histone H4 serves as a control for the template amounts. (M) Listing of analyzed genes, which were found to be unaffected (sample size ≥20 embryos/gene). N=2–4 independent experiments. |
