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Fig. 2. Xnr-1 expression in lateral plate (LP) grafts performed at
stage 20. Embryos in A–C were fixed and assayed for Xnr-1 expression
by in situ hybridization at stage 24/25. Embryos are photographed
from a dorsal view with anterior at the top of the page. Photos are
cropped to show relevant region only. Red arrows point to grafts, black
arrows to host Xnr-1 expression, and black arrowheads to Xnr-1
expression in the grafts. A: Left LP explant is grafted onto the right
side of host embryo. There is normal Xnr-1 expression in the host left
lateral plate mesoderm (LPM) and no Xnr-1 expression in the left LPM
grafted into the right side. B: Left LP explant grafted into the left side
of the host embryo as a control for the grafting experiment in A. There
is expression in both host left LPM and the graft. C: Right LP graft
expresses Xnr-1 when grafted into the left LP. The host left LPM
expresses Xnr-1 normally. D: Percentage of LP explants that express
Xnr-1 at stage 24 by in situ hybridization when grafted into the site
shown (grafts performed with donor and host at stage 20). |
