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Figure 4. Altered Xnr1 and Coco expression in Wnt11b manipulated embryos.Embryos were injected at the 4-cell stage into the DMZ and analyzed for Xnr1 (A–E) or Coco expression (F–J) by whole mount in situ hybridization. Wildtype expression patterns (A, F) were reduced (B, G) or absent/strongly reduced (C, H) in Wnt11b morphants and in embryos injected with a dnWnt11b DNA construct, while signal intensities were unaltered upon ectopic wildtype Wnt11b expression from a DNA construct (D, I). (E, J) Quantification of results. Note that co-injection of wild-type Wnt11b DNA was sufficient to partially rescue Xnr1 expression at the GRP of Wnt11b morphants. ***Very highly significant (p<0.001). a = anterior, l = left, p = posterior, r = right. |
