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Figure 4. Localization of the asymmetric gene Xnr-1 is affected by vibration during early, but not late, cleavage stages.
A) Control and vibrated embryos were examined at approximately st. 22 for the localization of Xnr-1 mRNA. All embryos were classified as having correct, left-sided expression (i), or incorrect right-sided (ii), bilateral (iii) or absent (iv) expression. Green arrows indicate correct expression, red indicate incorrect expression, and white indicate absent expression. All embryos are positioned with the anterior upward and the left (L) and right (R) sides are indicated. B) Quantification of incorrect Xnr-1 localization in control and vibrated embryos. When vibrations started at 1 cell, 2 cell, 4 cell or st. 6, there was a significant increase in incorrect Xnr-1 expression compared to controls (χ2 test, *p<<0.001). Vibrations starting at st. 8 were not statistically different from untreated controls. |
