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Fig. 5.
Depletion of Der protein on the left side randomizes the leftight axis. Embryos were injected at the four-cell stage with 2 ng Der MO in the dorsal left blastomere, and collected at stage 46 for phenotype, or 25 for marker expression. (A, B) Stage 46 embryos, ventral view. (A) Control, (B) Xnr1 MO injected (Situs inversus). Panels A1 and B1 show the normal head and trunk of the same embryos. The contour of the hearts is indicated, and arrows show the direction of the outflow tract of the heart and of gut looping. (C) Effect of Der MO on expression of Pitx2c (C, D) and Xnr1 (E, F). Left and right sides of the same embryos are shown. Embryos injected with Der MO are devoid of Xnr1 and Pitx2c expression in the anterior left LPM (D, F). |
