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nkx2-5xenopus   

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Experiment details for nkx2-5

Brade T et al. (2007) Assay

The amphibian second heart field: Xenopus islet-1 is required for cardiovascular development.

Gene Clone Species Stages Anatomy
nkx2-5.L laevis NF stage 17 to NF stage 18 cardiac mesoderm
nkx2-5.L laevis NF stage 25 cardiac mesoderm , heart primordium
nkx2-5.L laevis NF stage 28 cardiac mesoderm , heart primordium
nkx2-5.L laevis NF stage 29 and 30 cardiac mesoderm , heart primordium
nkx2-5.L laevis NF stage 33 and 34 myocardium , cardiac mesoderm , heart primordium

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  Fig. 3. Characterization of the spatial relationship between the Xisl-1 and Nkx2.5 expression domains. Single and double whole mount in situ hybridizations were performed to depict the expression domains of Xisl-1 and Nkx2.5 during early heart development in more detail. Pictures A, F, K, P show detection of Xisl-1 transcripts in light blue. Pictures B, G, L, Q show detection of Nkx2.5 transcripts in red. Pictures C, H, M, R show staining for both transcripts with the overlap of expression exhibiting a darker, rather purple staining. The asterisk behind a letter denotes images of identical embryos. Other embryos were stage matched. Pictures E, J, O depict schematics of the relative expression domains of Xisl-1 (blue dots) and Nkx2.5 (red lines) from embryos shown in pictures D, I, and N. (A–D) At neurula stages, both Xisl-1 and Nkx2.5 demarcate the cardiac crescent, which lies ventrally to the cement gland (arrows in pictures C, D). All embryos are shown from an anterior view. Picture D shows an enlargement of the cardiac crescent region of the embryo depicted in picture C. (F–I) At early tailbud stages, Xisl-1 and Nkx2.5 expression still overlaps largely in the heart region (arrow in picture H). (I) A ventral view of the heart region of an embryo to better demonstrate the overlapping expression of Xisl-1 and Nkx2.5 (arrow) during early tailbud stages. Xisl-1 only (blue) is also strongly expressed in the stomodeal region abutting the cement gland. (K–N) At stage 30 when the linear heart tube begins to form, Xisl-1 transcripts become restricted to a more anterior domain of the heart region (arrowhead) and the overlapping domain with Nkx2.5 decreases (black arrow). The white arrow points to the domain in which only Nkx2.5 is expressed. Picture N is a ventral view of the same embryo shown in picture M; head is to the left. The dotted lines highlight the Xisl-1 (white lines) and Nkx2.5 (black lines) expression domains. The ventral view of a stage matched embryo depicted in picture T shows a single staining for Xisl-1 in dark blue, in which the gap of Xisl-1 expression (asterisk) marks the region that expresses Nkx2.5 as seen in picture N. The arrow points to Xisl-1 expression in the posterior domain of the heart. (P–S) At progressive stages Xisl-1 transcripts are detected in the most anterior part of the heart region and its cardiac expression can only be documented in transverse sections. (U–X) Anterior to posterior series of transverse sections of a st. 28 embryo stained for Xisl-1 (purple) and Nkx2.5 (red). In the most anterior section (U) Xisl-1 transcripts are localized in two circular domains in the mesodermal layer (arrows) and in the pharyngeal endoderm (asterisk). No Nkx2.5 staining is detectable. (V) Along the A–P axis, expression of Xisl-1 becomes crescent-shaped in the mesoderm. (W) The section reveals an extensive overlap of Xisl-1 and Nkx2.5 in the cardiogenic mesoderm (arrows). (X) Further posterior, Xisl-1 becomes restricted to the dorsolateral domain (black arrows). In the ventralmost expression domain only Nkx2.5 transcripts are detected (white arrow). (Y–Z3) Transverse sections through the linear heart tube of st.30 embryos are shown. (Y, Y1) Nkx2.5 expression (red) throughout the heart tube (myocardium, mesocardium and pericardial roof) is depicted. A subsection of the mesocardium and pericardial roof is shown as an enlargement in picture Y1. (Y2, Y3) Nkx2.5 (red) and Xisl-1 (blue) expression is seen in the linear heart tube of a st.30 embryo. Xisl-1 transcripts are absent from the myocardium but are detected in the endocardium and in the foregut endoderm (asterisk). The Xisl-1 and Nkx2.5 expression domains overlap in the mesocardium and pericardial roof (see enlargement Y3). (Z, Z1) TnIc (red) is expressed in differentiated cardiomyocytes of the myocardium. Note the absence of TnIc transcripts in the mesocardium and pericardial roof. The enlargement shows a subsection of these domains (Z1). (Z2, Z3) TnIc (red) and Xisl-1 (blue) expression in the linear heart tube of a st.30 embryo. Note the complementary expression domains of TnIc and Xisl-1 from the myocardium, mesocardium, and pericardial roof, respectively. cg: cement gland; ec: endocardium; mc: myocardium; me: mesocardium; nf: neural fold; np: neural plate; pc: pericardial roof.

Gene Clone Species Stages Anatomy
nkx2-5.L laevis NF stage 28 heart primordium

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  Fig. 2. Spatial expression pattern of Xisl-1. (A) Xisl-1 is expressed in the anteriormost region of the embryo (shown from an anterior view, dorsal is up) including the cement gland anlage (asterisk) and the cardiac crescent (arrow). (B) During early neurula stages Xisl-1 expression extends dorsally adjacent to the neural folds (cephalic placodal area) (arrows). (C) Xisl-1 mRNA additionally marks the profundal–trigeminal placodal area. (D) A parasagittal section (anterior is to the left, dorsal is up) shows the expression of Xisl-1 in the anterior mesendoderm (arrow). (E) At neural tube stages Xisl-1 transcripts are present in the cardiac crescent (arrow) and are also detected in the stomodeum abutting the cement gland on the dorsal side (arrowhead). Xisl-1 expression is maintained in the cephalic placodes and their associated ganglia at least until st. 36 (E–K and data not shown). (F) Xisl-1 is expressed in the heart region (arrow). Punctate expression is seen in precursor cells of the developing vascular system (arrowheads). (G) Xisl-1 transcripts are abundant in the foregut endoderm and pharyngeal mesoderm and expression in the heart region has become restricted to an anterior domain (arrow); arrowhead points to pineal gland. (H) Co-staining for Xisl-1 (light blue) and Nkx2.5 (dark blue) shows that Xisl-1 is expressed more anteriorly compared to Nkx2.5. (I–K) Expression of Xisl-1 in the developing heart (asterisk) decreases and becomes restricted to a dorsolateral domain. aa: aortic arches; IFT: inflow tract; lpm: lateral plate mesoderm; np: neural plate; pPrV: profundal–trigeminal placodal area; gPr: profundal ganglion; gV: trigeminal ganglion; gVII: ganglia of facial nerve VII.