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Fig. 3. Loss of XHAPLN3 affects cardiogenesis. (A) Schema of bilateral injection experiment. Eight-cell-stage Xenopus embryos were bilaterally microinjected with 30 ng of XHAPLN3-MO. (B–M) Whole-mount in situ hybridization of Nkx2.5 and cardiac troponin I (cTnI). (B, C, J, K) Ventral views with anterior upwards. (D, E) Ventral views with anterior toward the left. (F, G) Lateral views with anterior toward the left and dorsal upwards. (H, I, L, M) Transverse sections of panels F, G, J, K embryos taken at respective positions of red lines. Dorsal upwards. Asterisk indicates archenteron. Scale bars in panels “B–G, J, K” and “H, I, L, M” indicate 0.5 mm and 0.1 mm, respectively. The number of photo-presenting phenotype per total number of injected embryos is indicated at upper right of each panel. |
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Fig. 6. Loss of Xhas2 affects cardiogenesis. Eight-cell-stage Xenopus embryos were bilaterally microinjected with 40 ng of Xhas2-MO. Whole-mount in situ hybridization of Nkx2.5 and cardiac troponin I (cTnI). (A, B, I, J) Ventral views with anterior upwards. (C, D) Ventral views with anterior toward the left. (E, F) Lateral views with anterior toward the left and dorsal upwards. (G, H, K, L) Transverse sections of panels E, F, I, J embryos taken at respective positions of red lines. Dorsal upwards. Asterisk indicates archenteron. Scale bars in panels “A–F, I, J” and “G, H, K, L” indicate 0.5 mm and 0.1 mm, respectively. The number of photo-presenting phenotype per total number of injected embryos is indicated at the upper right of each panel. |
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Fig. 4. Determining the stage of XHAPLN3-MO effect. (A) Schema of unilateral injection experiment. Eight-cell-stage Xenopus embryos were unilaterally microinjected with 15 ng of XHAPLN3-MO. (B-D) Whole-mount in situ hybridization of Nkx2.5. Arrowhead indicates the injected side. Nkx2.5 mRNA expression was unchanged at stage 17 (B), but decreased at stages 23 and 28 (C, D). (E) Experimental summary showing that XHAPLN3-MO had an effect from the early tailbud stage. |
