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Fig. 2. Developmental expression profiles of XHAPLN3 and Nkx2.5. (A) RT-PCR analysis of XHAPLN3 and Nkx2.5 expression during Xenopus development. Ornithine decarboxylase (ODC) served as a control. Reverse transcriptase negative (ODC (-RT)) reactions showed absence of genomic DNA contamination. (B–K, B′–K′) Whole-mount in situ hybridization of XHAPLN3 and Nkx2.5. Nkx2.5 expression indicates heart region. (B–E, J, K) Ventral views with anterior toward the left. (F–I) Lateral views with anterior toward the left and dorsal upwards. (B′–K′) Transverse sections of panels B–K embryos taken at respective positions of red lines in panels B–K. (B′–G′, J′, K′) Dorsal upwards. (H′, I′) Dorsal toward the left. so, somite; ov, otic vesicle; ea, eye anlage; PCM, precardiac mesoderm; EC, endocardium; MC, myocardium; A, atrium; BC, bulbus cordis; V, ventricle. Scale bars in B–K and B′–K′ indicate 0.5 mm and 0.1 mm, respectively. |
