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nkx2-5xenopus   

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Experiment details for nkx2-5

sfrp1 promotes cardiomyocyte differentiation in Xenopus via negative-feedback regulation of Wnt signalling.

sfrp1 promotes cardiomyocyte differentiation in Xenopus via negative-feedback regulation of Wnt signalling.

Gene Clone Species Stages Anatomy
nkx2-5.L laevis NF stage 18 cardiac progenitor cell
nkx2-5.L laevis NF stage 22 heart primordium
nkx2-5.L laevis NF stage 24 heart primordium

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  Fig. S2. Loss-of-function experiments show no change to cardiogenic gene expression during embryonic stages 18-24. (A-F) Whole-mount RNA in situ hybridisation analysis showing nkx2-5 expression at stages 18 (A,D), 22 (B,E) and 24 (C,F) in CoMO- injected (A-C) or sfrp1MO1-injected (D-F) embryos. (G-L) Whole-mount RNA in situ hybridisation analysis showing gata6b expression at stages 18 (G,J), 22 (H,K) and 24 (I,L) in CoMO-injected (G-I) or sfrp1MO1-injected (J-L) embryos.