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Fig. 8. Transverse sections through the heart-forming region of Xenopus embryos after double whole-mount in situ hybridisation for the two markers of cardiogenesis (in pale blue), Nkx2-5 (A–F) and MLC1av (G–K), in combination with POX2 (magenta). (A–C) Three representative sections marking progressively posterior slices through the same stage 24 embryo depicted in Fig. 7(B,C). (D–F) Three progressively posterior sections through the stage 26 embryo depicted in Fig. 7(D,E). Red arrows mark the ventral-most XPOX2-expressing mesodermal cells within the heart field. (G) Transverse section through the myocardial plate of a stage 30 embryo. (H–J) Three progressively posterior sections through the myocardium of a stage 32 embryo. (K) Transverse section through the looping heart tube of a stage 35 embryo. Sections (10 μm) are numbered (top right of each panel) commencing from the posterior limit of the cement gland (A–C and D–F) or the anterior limit of the myocardium (G–K). Ect, ectoderm; Mes, mesoderm; End, endoderm; Mc, myocardium; Ec, endocardium. |
