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FIG. 5. Expression of XNkx2-5/GFP transgene in Xenopus embryos. Normal and transgenic embryos were stained by wholemount in situ hybridisation for XNkx2-5 (A, E, G) or GFP (B, D, H) expression, respectively. Embryos were at early tail bud (stage 22, A, B), late tail bud (stage 27/28, E, F), and heart chamber formation
stages (stage 40, G, H). Transverse sections of embryos shown in A and B are shown in C and D, respectively, with expression shown
in dark blue and nuclei counterstained in red with Fuelgen. Black arrows indicate expression in the branchial arches. PE, pharyngeal endoderm; M, mesoderm; H, heart.
FIG. 6. The XNkx2-5 proximal promoter is not required for transgene expression. Normal and transgenic embryos at lateneurula or early tail bud stages were stained by whole-mount in situ hybridisation for XNkx2-5 (A, B) or GFP (C) expression,
respectively. Each pair of images shows lateral and ventral views of the same embryo. (A, B) Endogenous XNkx2-5 expression. (C, D) DNk2 transgene. (E, F) D659tK transgene. In all cases anterior is to
the left. |
