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Fig. 9. Tbx5 and Tbx20 synergistically act to regulate cardiac gene expression. (A-L) Embryos injected with the indicated morpholinos at the one-cell stage. (A-D) Nkx2-5 whole-mount in situ hybridization. (E-H) XANF whole-mount in situ hybridization. (I-L) XTnIc whole-mount in situ hybridization. (A,E,I) ControlMO, (B,F,J) TBX5MO injected at suboptimal dose, (C,G,K) TBX20MO injected at suboptimal dose, (D,H,L) TBX5MO and TBX20MO injected in combination at suboptimal doses. All embryo were cleared to reveal heart expression. (M) Statistics for embryos injected with suboptimal doses of TBX5MO and TBX20MO in combination with each other or with ControlMO. Hearts were judged as having defects if they displayed a pericardial edema, an unlooped heart tube or reduction in cardiac mass. |
