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Fig. 7. XPOX2 expression adjacent to the heart-forming region. Left-lateral views of Xenopus stage 20 (A), 24 (B) and 26 (D) embryos after double whole-mount in situ hybridisation for Nkx2-5 (pale blue) and POX2 (magenta). Higher magnification ventral views (C,E) of the heart field of the same embryos depicted in (B) and (D), respectively. Red arrow indicates expressing cells at the focal point located posterior of the cement gland (CG) on the ventral midline. (F) High magnification ventral view of the myocardial plate (Mc.P) of a stage 30 embryo after double whole-mount in situ hybridisation for MLC1av (pale blue) and POX2 (magenta). The stage 20 embryo (A) is a pigmented-wildtype example while the rest are albino embryos. A, anterior; P, posterior. |
