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Fig. S11. Effects of Hnf1β down- or upregulation on the expression of
various endodermal organ marker genes
Four-cell stage embryos were injected with RNA encoding β-galactosidase and either (A)
Hnf1β-morpholino or control-morpholino, or (B) RNA coding for Hnf1β-GR, vegetally into the
two dorsal blastomeres. At stage 36/37 embryos were used for WMISH against Pdx1
(pancreas, duodenum), hHex (liver), Nkx2.1 (thyroid, lung) and (B) Darmin (posterior
endoderm). |
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Fig. S12 Effects of Fzd4/Fzd4s downregulation on the expression of
various endodermal organ marker genes
Eight-cell stage embryos were injected with RNA coding for β-galactosidase and either Fzd4/
Fzd4s-morpholino or the corresponding mismatch-morpholino. At stage 38/39, embryos
were used for WMISH against Pdx1 (pancreas, duodenum), hHex (liver), Nkx2.1 (thyroid,
lung) and IFABP (stomach, intestine). |