|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
osr1
|
|
tropicalis
|
NF stage 15
|
endoderm
,
central endoderm
|
osr1
|
|
tropicalis
|
NF stage 28
|
stomodeal-hypophyseal primordium
,
intermediate mesoderm
,
endoderm
,
hindgut
,
alimentary system
,
[+]
|
osr1
|
|
tropicalis
|
NF stage 33 and 34
|
otic vesicle
,
brain
,
midbrain
,
hindbrain
,
spinal cord
,
[+]
|
osr1
|
|
tropicalis
|
NF stage 42
|
lung
,
alimentary system
,
esophagus
,
stomach
,
foregut
|
osr2
|
|
tropicalis
|
NF stage 15
|
endoderm
,
central endoderm
,
dorsal
|
osr2
|
|
tropicalis
|
NF stage 28
|
intermediate mesoderm
,
endoderm
,
hindgut
,
eye
,
foregut
,
[+]
|
osr2
|
|
tropicalis
|
NF stage 42
|
lung
,
alimentary system
,
esophagus
,
foregut
|
osr2
|
|
tropicalis
|
NF stage 33 and 34
|
otic vesicle
,
brain
,
spinal cord
,
pronephric duct
,
intermediate mesoderm
,
[+]
|
sftpc
|
|
tropicalis
|
NF stage 37 and 38
to
NF stage 42
|
endoderm
,
lung
,
foregut
|
|
|
Fig. 1. osr1 and osr2 are dynamically expressed in the developing Xenopus foregut and are required for lung development. (A-G′′) In situ hybridization of osr1 (A-D) and osr2 (E-H) expression in Xenopus tropicalis embryos and isolated gut tubes (D,H) at the indicated stages (st), anterior leftwards, dorsal upwards. Stage 15 embryos (A,E) are bisected and stage 33 embryos (C′-C[H11630],G′-G[H11630]) are sectioned to show the endoderm (outlined in yellow in A,E). fg, foregut; hg, hindgut; de, dorsal endoderm; pe, posterior endoderm; e, esophagus; l/t, presumptive lung and trachea; lv, liver; lpm, lateral plate mesoderm; k, kidney; s, stomach. (I-N′) surfactant protein c (sftpc) (I-L) and nkx2.1 (M,N′) expression in embryos injected on one side with Osr1/Osr2-MOs (10 ng each) or negative control mutated oligos MM-MO (20 ng) shows lung agenesis on the Osr1/Osr2-depleted side. (M’-N′) Sections. Numbers of embryos with the shown phenotype are indicated. Dorsal view of an isolated gut tube (K,L) shows the lung bud absent on the injected side (red arrow). |