|
Gene |
Clone |
Species |
Stages |
Anatomy |
neurog2
|
|
tropicalis
|
NF stage 11
|
ectoderm
,
dorso-lateral
,
lateral
|
neurog2.L
|
|
laevis
|
NF stage 11
|
|
neurog2
|
|
tropicalis
|
NF stage 12
|
trigeminal placode
,
neural plate
|
neurog2.L
|
|
laevis
|
NF stage 12
|
trigeminal placode
,
neural plate
,
dorso-lateral
|
neurog2
|
|
tropicalis
|
NF stage 15
|
chordal neural plate
,
trigeminal placode
,
Rohon-Beard neuron
,
olfactory region
|
neurog2.L
|
|
laevis
|
NF stage 15
|
chordal neural plate
,
trigeminal placode
,
otic region
|
neurog2
|
|
tropicalis
|
NF stage 20
to
NF stage 22
|
midbrain
,
hindbrain
,
spinal cord
,
eye
,
optic vesicle
,
[+]
|
neurog2.L
|
|
laevis
|
NF stage 22
|
midbrain
,
hindbrain
,
spinal cord
,
otic placode
,
trigeminal placode
,
[+]
|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
neurog1
|
|
tropicalis
|
NF stage 11
|
ectoderm
,
dorso-lateral
|
neurog1
|
|
tropicalis
|
NF stage 12
|
neural plate
,
dorso-lateral
,
anterior neural fold
,
Rohon-Beard neuron
,
neuron
|
neurog1
|
|
tropicalis
|
NF stage 20
to
NF stage 22
|
olfactory placode
,
trigeminal placode
,
optic vesicle
,
Rohon-Beard neuron
,
olfactory region
,
[+]
|
neurog1
|
|
tropicalis
|
NF stage 15
|
trigeminal placode
,
optic vesicle
,
dorso-lateral
,
Rohon-Beard neuron
,
neuron
,
[+]
|
neurog1
|
|
laevis
|
NF stage 11
|
ectoderm
,
dorso-lateral
|
neurog1
|
|
laevis
|
NF stage 12
|
trigeminal placode
,
neural plate
,
dorso-lateral
,
anterior neural fold
,
Rohon-Beard neuron
,
[+]
|
neurog1
|
|
laevis
|
NF stage 20
to
NF stage 22
|
spinal cord
,
otic placode
,
trigeminal placode
,
olfactory region
,
neural tube
|
neurog1
|
|
laevis
|
NF stage 15
|
trigeminal placode
,
dorso-lateral
,
Rohon-Beard neuron
,
neuron
|
neurog3
|
|
tropicalis
|
NF stage 12
to
NF stage 15
|
chordal neural plate
,
trigeminal placode
|
neurog3
|
|
tropicalis
|
NF stage 20
to
NF stage 22
|
hindbrain
,
midbrain-hindbrain boundary
,
spinal cord
|
neurog3
|
|
tropicalis
|
NF stage 15
|
chordal neural plate
,
dorso-lateral
|
neurog3
|
|
laevis
|
NF stage 12
|
chordal neural plate
,
dorso-lateral
|
neurog3
|
|
laevis
|
NF stage 11
|
ectoderm
|
neurog3
|
|
laevis
|
NF stage 22
|
hindbrain
,
midbrain-hindbrain boundary
,
spinal cord
|
|
|
Figure 2. Comparative in situ hybridization analysis of X. tropicalis Ngn1, Ngn2, Ngn3, and X. laevis Ngn1, Ngnr-1, and Ngn3 during early development. The antisense probes are indicated to the left. Shown is stage 11, dorsal view; stage 12, dorsal view, anterior up; stage 15 left panel, dorsal view, anterior up; stage 15, right panel anterior view; stage 20/22 left panel, lateral view; stage 20/22 right panel, anterior view. bp, blastopore; e, eye; hb, hindbrain; i, intermedial; l, lateral; m, medial; op, olfactory placode; otp, otic placode; tg, trigeminal-profundal placode. |
|
Display additional annotations [+]
|
|
Figure 3. A: Double in situ hybridization analysis of X. laevis Ngn1, Ngnr-1, Ngn3 (dark blue) with the mid-hindbrain boundary marker En-2 (red), marked by black arrowheads. Stages are indicated in the lower left corner, embryos are shown in an anterior view. B: Double in situ hybridization analysis of X. laevis Ngn1, Ngnr-1, Ngn3 (dark blue) with the marker for postmitotic neurons N-tubulin (red). The medial (m), intermediate (i) and lateral stripes (l) of N-tubulin expression are indicated in the transversal sections. |
|
Gene |
Clone |
Species |
Stages |
Anatomy |
neurog2
|
|
tropicalis
|
NF stage 29 and 30
|
brain
,
midbrain
,
hindbrain
,
spinal cord
,
ventricular zone
,
[+]
|
neurog2.L
|
|
laevis
|
NF stage 29 and 30
|
olfactory placode
,
forebrain
,
midbrain
,
hindbrain
,
spinal cord
,
[+]
|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
nkx2-1
|
|
laevis
|
NF stage 29 and 30
|
forebrain
,
ventral
|
tubb2b
|
|
tropicalis
|
NF stage 29 and 30
|
midbrain
,
trigeminal placode
|
tubb2b
|
|
laevis
|
NF stage 29 and 30
|
otic vesicle
,
retina
,
midbrain
,
trigeminal placode
|
neurog1
|
|
tropicalis
|
NF stage 29 and 30
|
olfactory placode
,
otic vesicle
,
midbrain
,
hindbrain
,
spinal cord
,
[+]
ventricular zone
,
pineal gland
,
trigeminal placode
,
epibranchial placode
,
lateral line placode
,
dorsal lateral line
,
middle lateral line
,
anterior
,
posterior
,
facial nerve
,
glossopharyngeal nerve
,
vagus nerve
|
neurog1
|
|
laevis
|
NF stage 29 and 30
|
otic vesicle
,
midbrain
,
hindbrain
,
pineal gland
,
trigeminal placode
,
[+]
|
en2
|
|
laevis
|
NF stage 29 and 30
|
midbrain-hindbrain boundary
|
neurog3
|
|
tropicalis
|
NF stage 29 and 30
|
hindbrain
,
spinal cord
|
neurog3
|
|
laevis
|
NF stage 29 and 30
|
hindbrain
,
spinal cord
|
|
|
Figure 4. A: Comparative in situ hybridization analysis of X. tropicalis Ngn1, Ngn2, Ngn3, and X. laevis Ngn1, Ngnr-1, and Ngn3 in stage 30 embryos. Probes are indicated on the left of each line of embryos. Shown: first panel, lateral view, anterior to the left; second panel, close-up of the head region; third panel dorsal view of head region; fourth panel, transverse section at the level of the retina; fifth panel, transverse section at the level of the otic vesicle. The transversal sections were performed with embryos stained for both Ngn (dark blue) and N-tubulin (red). VII, facial epibranchial placode; IX, glossopharyngeal epibranchial placode; X1, first vagal epibranchial placode; X2, second vagal epibranchial placode; X3, third vagal epibranchial placode; hb, hindbrain; mb, midbrain; nc, notochord; op, olfactory placode; vOT, otic vesicle; tg, trigeminal placode; pg, pineal gland; pP, posterior lateral line placode; pM, middle lateral line placode; pAD, anterodorsal lateral line placode; r, retina. B: Double in situ hybridization of stage 30 X. laevis embryos with Ngn1, Ngnr-1, Ngn3 (dark blue) and the mid-hindbrain boundary marker En-2 (red) or with the ventral forebrain marker Nkx2.1 (red) (transversal section). Black arrowhead marks the expression of Ngn3 in the ventral forebrain. |
|
Gene |
Clone |
Species |
Stages |
Anatomy |
neurog2.L
|
|
laevis
|
NF stage 29 and 30
|
olfactory placode
,
otic vesicle
,
brain
,
midbrain
,
hindbrain
,
[+]
spinal cord
,
ventricular zone
,
pineal gland
,
trigeminal placode
,
epibranchial placode
,
dorsal lateral line
,
middle lateral line
,
anterior
,
facial nerve
,
glossopharyngeal nerve
,
vagus nerve
|
|
|
Fig. 4. Neurog2 expression at stg. 30 |
|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
ptf1a
|
|
laevis
|
NF stage 41
|
pancreas
|
neurog1
|
|
tropicalis
|
NF stage 41
to
NF stage 43
|
forebrain
,
telencephalon
,
diencephalon
,
spinal cord
,
nervous system
|
neurog1
|
|
laevis
|
NF stage 41
to
NF stage 43
|
otic vesicle
,
forebrain
,
midbrain-hindbrain boundary
,
spinal cord
|
neurog3
|
|
tropicalis
|
NF stage 41
to
NF stage 43
|
hindbrain
,
spinal cord
|
neurog3
|
|
laevis
|
NF stage 41
to
NF stage 43
|
hindbrain
,
spinal cord
,
stomach
,
intestine
|
|
|
Figure 5. A: Comparative in situ hybridization analysis of X. tropicalis Ngn1, Ngn2, Ngn3, and X. laevis Ngn1, Ngnr-1, and Ngn3 in stage 41/43 embryos. Probes are indicated on the left. Left panel shows a lateral view of the embryos, right panel shows a dorsal close up of the head region. Red arrowheads indicate the ventral Ngn3 expression in intestine B: Transverse section showing X. tropicalis Ngn2 expression in the ciliary marginal zone (CMZ) of the retina. C: Double in situ hybridization of analysis of X. laevis Ngn3 (dark blue) and Ptf1a/p48 (red) in stage 41 gut explants. Red arrowheads mark stained enteroendocrine cells. duo, duodenum; int, intestine; lt, lung tube; lv, liver; oes, esophagus; pa, pancreas, pr, proctodeum; st, stomach. |