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FIG. 2. Neural tissue induced from ectoderm transplants. Each row shows a case in which FLDx-labeled early gastrula ectoderm was
transplanted to a neural plate stage embryo (as shown in Fig. la). In each row, the left figure shows a section viewed under differential
interference contrast (DIC), the middle figure shows FLDx fluorescence, and the right figure shows immunofluorescence staining with anti-NCAM
antibody. Note that both the host neural tube and host eye stain strongly for N-CAM. Abbreviations: e - eye, nt = neural tube, p
= pharynx. Scale bar = 200 Um. A-C: Transplant to the dorsolateral head region. Transplanted tissue (labeled tissue in B, marked by arrow) has
fused to the host eye and stains with anti-N-CAM (arrow in C). D-F: Transplant to the lateral head area. The transplant (labeled tissue in E,
marked by arrow) is located ventral to the level of the eye, but has still made a small neural-tube-like structure that stains positively for N-CAM
(arrow in F). G-I: Transplant to anterior ventral ectoderm. The transplant (labeled tissue in H, marked by arrow) is located in the region of the
ventral midline, and has formed no neural tissue, as judged by the absence of N-CAM staining of this tissue (I). |
