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Fig. 3. Injection of a dominant
negative form of ZFPIP (ZFPIPp)
perturbed neural markers. The xZFPIPp
transcripts were injected in one
blastomere of two cell-stage embryos.
The correct injection of xZFPIPp
mRNAs were checked by whole mount
hybridization using xZFPIPp as
riboprobes (a). The correct translation
of the protein was checked by Western
blots carried out with a-ZFPIP
antibodies on extracts prepared from
injected and non-injected stage 22
embryos (b). Whole mount hybridizations
of late neurula embryos using
N-cam, en2 or krox-20 riboprobes
demonstrated an altered expression of
N-cam and en2 expression in injected
blastomeres. The side injected with
xZFPIPp capped mRNAs and the
non-injected side are visualized by
b-galactosidase staining and are
indicated respectively by * and ** (c). |
