| |
Fig. 2. Distribution of N-CAM and NC-1 immunoreactivity in the nerves. Section of premetamorphic Xenopus tadpoles
(stage 52 NF) (A,B) and of animals toward the end of the metamorphic climax (stage 64 NF) (C-F) were double stained
with polyclonal antibodies against Xenopus N-CAM (A,C,E) and with NC-1 monoclonal antibody (B,D,F). Before
metamorphosis all nerves were intensely stained by anti-N-CAM and NC-1 antibodies. During metamorphosis the staining of
anti-N-CAM antibodies was greatly reduced in peripheral nerves (C) but remained high in central nerves such as the optic
nerve (E); NC-1 staining persisted in the nerves throughout metamorphosis, dm, dorsal muscle; m, muscle; on, optic nerve;
sn, sciatic nerve. Bar: 100um. |
