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Figure 4. mZac1c-induced retinal ganglion cell layer (GCL) clusters have a neuronal identity. A�P: Stage 40 green fluorescent protein�Zac1 (GFP+Zac1) -injected retina showing GFP epifluorescence (green, A�C,E�G,I�K,M�O) and immunolabeling with the pan-neuronal markers nerve cell adhesion molecule (NCAM, red, B�D), neurofilament (NF, red, F�H), Xen-1 (red, J�L), and Zn12 (red, N�P). C�D, G�H, K�L, O�P are high-magnification images of the boxed areas in B, F, J, N, respectively. Arrowheads in L and P mark disorganized inner plexiform layer. le, lens; gcl, ganglion cell layer; inl, inner nuclear layer; ipl, inner plexiform layer; onl, outer nuclear layer. |
