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Fig. 1. Nanos1-depleted Xenopus embryos are deficient in PGCs. (A) Nanos1-MO blocks the translation of endogenous nanos1. Confocal analysis showing the expression of Nanos1 (red) and Xiwi (germ plasm marker, green) at the eight-cell stage of embryos previously injected with Nanos1-Ctrl-MO or Nanos1-MO at the one-cell stage. Uninjected embryos served as controls. Arrows indicate Nanos1 signal. (B) Knockdown of Nanos1 results in loss of PGCs. WISH Xpat analysis of stage 37/38 wild-type (WT), Nanos1-Ctrl-MO- and Nanos1-MO-injected embryos. For the rescue, Nanos1-MO embryos were injected with nanos1-mut RNA. (C) Summary of PGC loss at different stages after Nanos1 knockdown. P-values by one-way ANOVA. Error bars indicate s.e. (D) Hematoxylin and Eosin-stained sections of gonads from WT (stage 54), Nanos1-Ctrl-MO (stage 55), Nanos1-MO (stage 54) and rescued (stage 54) embryos, Arrows indicate germ cells. Note that nanos1 morphants were completely rescued by nanos1-mut RNA injection. Scale bars: 130 μm in A; 100 μm in B; 20 μm in D. |
