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Figure 2. Tissue localisation of Hoxc6 isoforms in cross-sections. A: SF expression in the somitic tissue and neural tube. B: Expression of LF in somitic tissue and neural tube; in addition, LF is expressed in the pronephric anlage (arrowheads). C, D: Close-ups of the neural tubes shown in A and B. Expression of the Hoxc6 isoforms is detected in all cells of the neural tube with exception of the floorplate. E: MyoD expression in the somites. F: Xlim-1 expression in the neural tube and in the pronephric anlage (arrowheads). G: FoxF1 expression in the lateral plate mesodem. H: Position of the shown sections within stage-27 embryos, and localization of different tissues in the sections. Neural tube (blue); somite mesoderm (red); notochord (brown); endoderm (yellow); lateral plate mesoderm (dark orange); the pronephric anlage (light orange). I: Cross-section at the level of the branchial arches (br) as indicated in the inlay. Expression of SF is found in the neural tube (ne), even though it was not visible in whole mount in situ hybridization. J: Expression of LF in the neural tube at the same level. |
