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Fig. 3. Tsg and Bmp7 cooperate in ventral development in Xenopus embryos. (A,F,K,P) Control and morpholino-injected embryos at stage 12 hybridized with sizzled probe. Expression of sizzled is reduced in Bmp7-MO- or Tsg-MO-injected embryos and almost completely lost in the Bmp7-MO/Tsg-MO-injected embryos. (B,G,L,Q) Expression of Otx2 in injected embryos at stage 13; there is an increase in Bmp7-MO/Tsg-MO-injected embryos. (C,H,M,R) Embryos at stage 18 hybridized with Otx2 and Myod1 probes. The expansion of Otx2 regulates and is no longer seen at this stage. (D,E,I,J,N,O,S,T) Phenotypes of morpholino-injected embryos at (D,I,N,S) stage 39 (2 day) and (E,J,O,T) stage 42 (3 day). Bmp7-MO-injected embryos have a reduced ventral fin (arrowhead); Tsg-MO-injected embryos have a bent tail and reduction of ventral fin. Co-injection of Bmp7-MO and Tsg-MO synergize, causing a severe loss of ventral fin (arrow in S) and at later stages truncation of the entire tail region (T). |
