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Figure 3. Patterning and morphology of the presomitic mesoderm in embryos injected with the intg alpha 5 MO. A-D: In situ hybridization with a probe for MyoD in a stage-19 (A,B) and a stage-25 (C,D) embryo, showing uninjected (A) and intg alpha 5 MO-injected (B) sides of an embryo, and control MO injected (C) and intg alpha 5 MO-injected embryos (D) Arrows in D indicate individual somites. E,F: Immunofluorescence staining for beta -catenin (E, red in F) and GFP (green in F) showing normal morphology of the presomitic mesoderm in cells exposed to the intg alpha 5 MO (GFP positive cells, bottom). E: Inverse image is shown to highlight cell borders. Asterisks in E mark somites in the process of turning. Anterior is on the left in all images. Scale bar = 100 mu m. |
