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myod1xenopus   

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Experiment details for myod1

The myocardin-related transcription factor, MASTR, cooperates with MyoD to activate skeletal muscle gene expression.

The myocardin-related transcription factor, MASTR, cooperates with MyoD to activate skeletal muscle gene expression.

Gene Clone Species Stages Anatomy
myod1.S laevis NF stage 14 presomitic mesoderm , paraxial mesoderm
myod1.S laevis NF stage 22 to NF stage 32 presomitic mesoderm , somite
myod1.S laevis NF stage 37 and 38 to NF stage 39 somite , myotome , hypaxial muscle , epaxial muscle , jaw muscle

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  Fig. 1. MASTR is a previously uncharacterized member of the myocardin-related family of proteins. (A) Alignment illustrating the domains of the four known Xenopus myocardin family proteins and also mouse MASTR. Percentages indicate identity within each domain relative to myocardin or between Xenopus and mouse MASTR. (B) Co-IP experiments demonstrate that MASTR forms a physical complex with SRF. HA-tagged SRF and radiolabeled in vitro-translated MASTR, δMASTR, and EGFP are shown (Left). Only full-length MASTR protein coprecipitated with SRF (Right). (C–F). In situ hybridization analysis of MASTR expression during Xenopus development. MASTR transcripts are detected specifically in skeletal muscle tissues at all stages. Expression in hypaxial muscle (hy) and jaw muscle (jm) is indicated. (G–J) Transcriptional expression of MyoD. Intensity of MyoD expression differs from MASTR, but the location of transcripts is identical. (K–N) Expression of the striated muscle marker, cardiac α-actin. In addition to marking all tissues expressing MASTR, cardiac α-actin is expressed in the heart (h). (O) RT-PCR analysis showing MASTR expression is first detected in the gastrula (St 10.5) coincident with the appearance of MyoD and Myf5 mRNA. The muscle differentiation marker cardiac α-actin is not detected until St 12.5. Ornithine decarboxylase (ODC) was used as a loading control. (P) RT-PCR analysis of MASTR transcripts in adult frog tissues. MASTR is expressed in representative skeletal muscles (abdominal rectus, quadriceps, and soleus) but not in heart, smooth muscle (intestine), or other tissues.