| |
Fig. 2. RGS4 RNA injection at the 2-
cell stage perturbs skeletal muscle
and retinal development.
(A,B) Immunocytochemical staining
of skeletal muscle in a stage-28
embryo injected with control fs-
RGS4 RNA (A) or with wild-type
RGS4 RNA (B). The section was
stained with the muscle-specific
antibody 12/101 (Kintner and
Brockes, 1984), and bound antibody
was visualized with a red color
substrate. The section was then
stained with Hematoxylin. Arrows
indicate skeletal muscle.
(C,D) Histological section through
the eye of a stage-28 embryo injected
with control fs-RGS4 RNA (C) or
wild-type RGS4 RNA (D) and
stained with Hematoxylin. Note the
infoldings of the pigmented (black)
presumed retinal cells in the wildtype
RGS4 RNA-injected embryo.
(E,F) Expression of XmyoD in
gastrula-stage embryo injected with
control fs-RGS4 RNA (E) or wildtype
RGS4 RNA (F) and visualized
by whole-mount in situ
hybridization. (G,H) Expression of Xbra in gastrula-stage embryo injected with control fs-RGS4
RNA (G) or wild-type RGS4 RNA (H) and visualized by whole-mount in situ hybridization. |
