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Figure 1. Zic1 regulates the expression of direct target genes in whole embryos. The expression patterns by in situ hybridization are shown. A-C: aqp-3b. D-F: cpl-1. G-I: CRABP-II. J, K: tnrc4. L, M: Xl.25952. N, O: Xl.8933. P, Q: myoD. A, D, G, J, L, N, and P are uninjected embryos illustrating the expression of aqp-3b, cpl-1, CRABP-2, tnrc4, Xl.25952, Xl.8933, and myoD, respectively. B, E, H, K, M, O, and Q show reductions in gene expression produced by injection of RNA for a dominant interfering construct of zic1 (zic1 Delta N, previously called dnzic1; Li et al.,[2006]; Merzdorf and Sive,[2006]). C, F, and I show increases in gene expression resulting from injection of full-length zic1 RNA. One cell of 2-cell embryos was injected with either 100 pg zic1 RNA or 100 pg zic1 Delta N RNA together with 25 pg lacZ RNA. Stage 15-18 neurula stage embryos are shown. Arrowheads mark the injected sides. |
