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Fig. 6. (A) Western blot of late gastrula embryos injected into the two blastomeres at the two-cell stage with 300 pg of MyoDF, 100 pg of MEF2AF, MEF2DF and MEF2CF mRNA. Proteins were detected with anti-flag antibody. (B) Embryos were injected unilaterally at the two-cell stage at the level of marginal zone with the quantities of mRNA described for western blot. Embryos were fixed at stages 18–19 and submitted to whole-mount in situ hybridization with desmin probe. The injected mRNAs are indicated in each image. mRNA encoding the lineage tracer β-galactosidase (blue) was used to identify the injected side of embryos (e–g) except for embryos injected with synthetic mRNA of MyoD where the ectopic expression of desmin is easily detected (a–d). |
