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FIGURE 6. BMP signaling is required for liver development. (A) Design of the experiment. Lineage tracer was injected (B) alone or (C–F) with ∼30 pg/blastomere of truncated BMP Receptor (tBR) mRNA in dorso-vegetal blastomere D1 at the 32-cell stage. Heart and liver were revealed by double WMISH of myl7 (turquoise) and nr1h5 (purple). (C–F) four examples showing attenuation of liver fate specification in vivo following localized BMP inhibition by tBR (red-pink; pointed by arrows). N = 11, all showing effect on nr1h5 expression. Ventral views are shown, anterior is up. BMP signaling inhibition attenuates liver cell fate specification in Gata4 injected AC. (G) qPCR analyses of st. 34 explants show downregulation of nr1h5 as a consequence of BMP inhibition via tBR. At st. 10, tBR has no effect on the ability of Gata4 to induce sox17 but reduces hhex induction. (H) Treatment of Gata4-expressing AC explants with 30 μM dorsomorphin (DM) leads to downregulation of nr1h5. |
