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Fig. 1. (A) Expression of Xmyf-5 at the gastrula and neurula stages. Expression of Xmyf-5 was assayed in albino embryos by mRNA in situ hybridisation at the early gastrula/stage 10.5 (i) and late neurula/stage 19 (ii). (B) Schematic representation of XTM1, a 7.28 kb genomic fragment containing the X. tropicalis myf-5 gene. XTM1 is composed of 3 exons of 594 bp, 76 bp and 441 bp (untranslated regions shown in grey and coding sequence in red) separated by introns of 548 bp and 1445 bp. The start of transcription is indicated by an arrow. The percent similarities of the Xtmyf-5 exons with Xlmyf-5 are shown below each exon. A X. tropicalis myf-5-specific in situ hybridisation probe was raised to the third exon. (C) The expression of XTM1 in transgenic X. laevis embryos. (iii) Non-transgenic X. tropicalis embryo at stage 10.5 stained with species-specific Xtmyf-5 probe. (iv, v) Non-transgenic albino (iv) and pigmented (v) stage 10.5 X. laevis embryos stained with the species-specific Xtmyf-5 probe; note lack of staining. (vi, vii and viii) XTM1 transgenic X. laevis embryos at stage 10.5 (vi and vii) and stage 19 (ix), stained with the species-specific Xtmyf-5 probe. Note that the embryo in vii is half-transgenic, demonstrating the specificity of the XTM1 probe. Gastrula embryos are orientated with the dorsal axis pointing up and vegetal pole facing out. The neurula stage embryos are shown with the dorsal axis pointing out and anterior to the left. |
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Fig. 7. (A) Endogenous expression of Xvent-1 and Xmyf-5. (B) The effect of Xvent-1 mis-expression on Xmyf-5. Single blastomeres of 4 cell stage embryos were injected with 250 pg Xvent-1 capped mRNA along with the lineage tracer β-gal (shown in blue). Xmyf-5 expression was then assayed at stage 10-11 by mRNA in situ hybridisation (purple). Embryos are orientated with dorsal pointing up and vegetal pole facing out. |
