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Figure 3. The DML is invaded by paraxial mesoderm and neuroectoderm in FoxA4 morphants.
Dorsal views of embryos at neural plate stage hybridised with myf5 (A, D), myoD (B, E, G, H), or sox2 probes (C, F). They were injected before the first cleavage with 20 ng CtrlMO (A–C) or of FoxA4MO (D–F), 0.25 ng of foxA4FL mRNA (H), or left uninjected (G). In FoxA4 morphants, myf5 (D) and myoD expression (E) was found in the DML (40%, n = 23; 56%, n = 23, respectively). The left embryo in E shows that myoD expression invaded the DML along the A–P axis. The right embryo in E shows stretches of the myoD domains fused at the midline (arrowheads). Sox2 transcripts obliterated the prospective FP (79%, n = 19) (F). In embryos injected with foxA4FL mRNA, myoD expression was reduced, and the medial borders of the bilateral domains were more separated (H, 42%, n = 26) than in control siblings (G).
doi:10.1371/journal.pone.0110559.g003 |
