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Fig. 2. Morpholino-mediated knock-down of Klhl31 results in formation of a thickened myotome. Control morpholino (A–D), translation start site morpholino Klhl31 MO (E–H) or a splice morpholino (I–L) was electroporated into somites, the electroporated side is indicated with an asterisk (*). Contralateral somites serve as internal control. After 48 h embryos were subjected to in situ hybridization with Pax3, MyoD, Myf5 or myogenin (purple), as indicated. MOs were detected using anti-FITC antibody coupled to alkaline phosphatase (red). Some Fast red signal is lost after sectioning procedure. A thickened dermomyotome and myotome are evident after Klhl31 knock-down. Area of expression is presented below each panel. Neural tube, nt; notochord, nc; dermomyotome, dm; and myotome, my. |
