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myf5xenopus   

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Experiment details for myf5

Xenopus Myf-5 marks early muscle cells and can activate muscle genes ectopically in early embryos.

Xenopus Myf-5 marks early muscle cells and can activate muscle genes ectopically in early embryos.

Gene Clone Species Stages Anatomy
myf5.L laevis NF stage 11.5 presumptive paraxial mesoderm
myf5.L laevis NF stage 18 mesoderm , presomitic mesoderm , somite , paraxial mesoderm

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  Fig. 4. XMyf5 transcripts are somite-specific. In situ hybridizations to transverse sections through the posterior parts of late (stlli) gastrulae (A,B) and late (stl8) neurulae (C,D), probed for XMyf5 (A,C) or XMyoD (B,D). Note that the neurula sections are from the 'tail' region used for the northern analysis in Fig. 5 below, and show somite-specific expression in this part of the embryo which was not dissected into its component tissues. The gastrula sections, from wild-type embryos, were bleached with hydrogen peroxide (see Materials and methods); the neurula sections were from albino embryos. Probe concentrations (and exposure times) were: (A,B) 400ctsmin~'^l~1 (one month); (C) TSOctsmin'Vl1 (one week); (D) V"1 (two weeks).