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Fig. 4. XMyf5 transcripts are somite-specific. In situ hybridizations to transverse sections through the posterior parts of late
(stlli) gastrulae (A,B) and late (stl8) neurulae (C,D), probed for XMyf5 (A,C) or XMyoD (B,D). Note that the neurula
sections are from the 'tail' region used for the northern analysis in Fig. 5 below, and show somite-specific expression in this
part of the embryo which was not dissected into its component tissues. The gastrula sections, from wild-type embryos, were
bleached with hydrogen peroxide (see Materials and methods); the neurula sections were from albino embryos. Probe
concentrations (and exposure times) were: (A,B) 400ctsmin~'^l~1 (one month); (C) TSOctsmin'Vl1 (one week); (D)
V"1 (two weeks). |