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Fig. 5. Activation of muscle markers in embryos lacking REEP4 is normal during neurula stages but their expression becomes disorganised or reduced thereafter. X. laevis (A-BB) or X. tropicalis (CC-FF) embryos were injected, respectively, with 90 ng MO or 30 ng MO2. Control embryos received the same amounts of control MO. They were cultured to neurula or tailbud stages and analysed by in situ hybridisation for expression of Myf5 MyoD, Mrf4, the 12/101 epitope, Dystrophin, Cardiac actin and Myosin Heavy Chain. Note in X. laevis that expression of markers at the neurula stage is normal (A,B; E,F; U,V; Y,Z) but that they become disorganised (C,D; G,H; K,L; O,P; S,T) or reduced (W,X; AA,BB) thereafter. The decrease in Cardiac actin expression in the heart in (X) is not a consistent observation. Expression of the 12/101 epitope in X. tropicalis is more sensitive to loss of REEP4 function than is expression in X. laevis. Note the decreased levels of 12/101 staining in (DD) compared with (P). |
