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Fig. 2. Time course of pFLCFP reporter gene expression in transgenic embryos. Transcripts of the endogenous Xmyf-5 or the GFP reporter gene were detected by whole mount in situ hybridization with Xmyf-5 or GFP antisense RNA probes, respectively. (A–E) Expression pattern of endogenous Xmyf-5 RNA at (A) stage 10−, (B) stage 11, (C) stage 11.5, (D) stage 19, (E) stage 31; (F–J) expression of the pFLGFP transgene in pigmented embryos at the same stages. The expression pattern of GFP transgene was very similar to that of the endogenous myf-5, although a faint positive signal was detected above the dorsal lip in wide type embryos (A), but not in transgenic embryos at stage 10− (F). (A–C,F–H) Vegetal views of gastrulae with the dorsal side facing up; (D,I) dorsal views of neurulae; (E,J) lateral views of tadpoles. Neurulae and tadpoles: anterior to the left. |