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mycxenopus heart 

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Experiment details for myc

Rodrigues CO et al. (2008) Assay

A Myc-Slug (Snail2)/Twist regulatory circuit directs vascular development.

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Gene Clone Species Stages Anatomy
myc.L laevis NF stage 35 and 36 heart

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  Fig. 1. Xc-Myc expression and morpholino knockdown. (A) In situ hybridization of Xc-Myc expression during development. (i) Gastrula: expression in involuting mesoderm (M) that flanks the yolk plug. (ii) Neurula: expression in anterior region and in the lateral edges of neural crest (NC). (iii) Early tailbud: expression in the developing eye (E) and migratory neural crest cells (MNC). (iv) Tailbud (∼stages 27/28): expression in eyes (E), brain (B), somites (S), pharyngeal arches (PA) and ventral blood islands (VBI). (v) Stage 35/36: expression is predominant in head and heart region (eyes, E; brain, B; heart, H; rostral lymph sac, RLS; aortic arches, AA). (B) The Xc-Myc morpholino blocks translation of Xc-Myc. In vitro translation of transcripts for wild-type (Xc-Myc) or a morpholino-resistant mutant (Xc-Myc-Mut) in the absence (-) or presence (+) of Xc-Myc morpholino (Mo) is shown. Control morpholinos were non-specific (A) or a scrambled Xc-Myc (B) morpholino. (C) Knockdown of Xc-Myc protein expression in vivo at different stages. Western blot of Xc-Myc expression in embryos injected with Xc-Myc morpholino (Mo); uninjected (uninj); loading control (input).

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Gene Clone Species Stages Anatomy
myc.L laevis NF stage 37 and 38 heart

  Fig. 3. Xc-Myc knockdown impairs endothelial cell development. (A,B) Whole mount in situ hybridization (stage 37) for endothelial lineage marker X-msr; (B) enlargement of the embryos shown in A. X-msr staining is reduced and diffuse in Xc-Myc knockdown embryos throughout, and the vitelline vein network (vvn), aortic arches (aa), tunica vasculosa lentis (tvl), anterior cardinal vein (acv) and rostral lymph sac (RLS) are reduced or missing. H, heart; pcv, posterior cardinal vein; isv, intersegmental vessels. (C) Histology of posterior cardinal vein (pcv) and dorsal aorta at stage 37. Uninj, uninjected control; Xc-Myc Mo, Xc-Myc morpholino-injected embryos. The vessels found in Xc-Myc morpholino-injected embryos have remarkably thin walls and empty lumens. Analyses based on five or six embryos from two different experiments.