|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
fzd4
|
|
tropicalis
|
NF stage 15
|
pre-chordal neural plate
,
eye primordium
,
optic vesicle
,
neural tube
|
hesx1
|
|
tropicalis
|
NF stage 13
to
NF stage 21
|
pre-chordal neural plate
,
eye primordium
,
optic vesicle
,
optic field
|
hesx1
|
|
tropicalis
|
NF stage 15
|
pre-chordal neural plate
,
eye primordium
,
optic vesicle
|
vsx1
|
|
tropicalis
|
NF stage 15
|
pre-chordal neural plate
,
eye primordium
,
optic vesicle
|
myb
|
|
tropicalis
|
NF stage 15
|
pre-chordal neural plate
,
eye primordium
,
optic vesicle
|
foxn4
|
|
tropicalis
|
NF stage 15
|
pre-chordal neural plate
,
eye primordium
,
optic vesicle
|
mab21l2
|
|
tropicalis
|
NF stage 15
|
pre-chordal neural plate
,
eye primordium
,
optic vesicle
,
presumptive midbrain-hindbrain boundary
|
fezf2
|
|
tropicalis
|
NF stage 13
to
NF stage 21
|
pre-chordal neural plate
,
eye primordium
,
optic vesicle
,
optic field
|
|
|
Fig. 7. Genes with altered expression in rax mutants include those necessary for retina formation and forebrain patterning. (A) Table listing key up-regulated (red) and down-regulated (blue) genes from RNA-Seq analysis. (All significantly affected genes identified in this analysis can be found in Table S4) (B) In situ hybridization for up-regulated genes in St. 15 embryos. White arrows indicate regions where up-regulation is observed in mutants in the presumptive retinal field. (C) In situ hybridization for down-regulated genes in St. 15 embryos. White arrows indicate regions in the eye field where expression is not detected in the mutant. (D) vsx1 expression (arrows) at neural plate stage is reduced in the heterozygote compared to wildtype and not detected in rax homozygous mutant embryos. (E) A time course of fezf2 and hesx1 expression from late gastrula (St. 12) through neural tube stage (St. 21) in rax mutant and wildtype embryos. White arrows indicate regions where repression is seen in wildtype embryos and not observed in rax mutants. |