|
Gene |
Clone |
Species |
Stages |
Anatomy |
mxi1.L
|
|
laevis
|
NF stage 10.5
|
ectoderm
,
dorsal
|
mxi1.L
|
|
laevis
|
NF stage 11.5
|
chordal neural plate
|
mxi1.L
|
|
laevis
|
NF stage 12.5
|
chordal neural plate
|
mxi1.L
|
|
laevis
|
NF stage 13
|
chordal neural plate
,
trigeminal placode
|
mxi1.L
|
|
laevis
|
NF stage 14
|
chordal neural plate
,
trigeminal placode
|
mxi1.L
|
|
laevis
|
NF stage 16
|
neuroectoderm
,
chordal neural plate
,
sensorial layer of neurectoderm
|
mxi1.L
|
|
laevis
|
NF stage 22
|
olfactory placode
,
hindbrain
,
optic vesicle
,
neural tube
|
mxi1.L
|
|
laevis
|
NF stage 27
|
olfactory placode
,
midbrain
,
hindbrain
,
optic vesicle
|
mxi1.L
|
|
laevis
|
NF stage 37 and 38
|
retina
,
midbrain
,
hindbrain
,
ventricular zone
,
optic vesicle
|
Display additional annotations [+]
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Fig. 1. (A) Comparison of the predicted amino acid sequence of Xmxi1 with the mouse Mxi1 isoforms. GenBank accession number Xmxi1: DQ137875. (B) RT-PCR analysis of Xmxi1 at different stages of development. Histone H4 was used as a loading control. (C) Whole-mount in situ hybridization expression analysis of Xmxi1 in comparison with X-ngnr-1. (D–F) Double labeling of Xmxi1 and Sox3 expression. Sox3, red; Xmxi1, dark blue. (D) Stage 14, anterior view. (E) Stage 14, dorsal view. White arrowhead marks the lateral stripe of Xmxi1. (F) Transversal section of E. (G–N) Spatial expression of Xmxi1 during later stages of Xenopus development. (G) Stage 13, dorsal, anterior up. (H) Stage 16, dorsal view, anterior left. (I) S1 transversal section. (J) Stage 22, lateral. (K) Stage 27, anterior (L) Stage 37, lateral. (M) S2 transversal section. (N) S3 transversal section, dark brown staining at the roof of the spinal chord is pigmentation not Xmxi1 staining. el, epithelial layer; hb, hindbrain; i, intermediate; iz, intermediate zone; l, lateral; le, lens; m, medial; mb, midbrain; n, notochord; op, olfactory placode, r, retina; sl, sensory layer; st, stage; tg, trigeminal placodes; vz, ventricular zone. |
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Display additional annotations [+]
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Fig. 2. (A–J) Regulation and requirement of Xmxi1 during primary neurogenesis. Whole-mount in situ hybridization of stage 14 embryos injected with Sox3 (50 pg), X-ngnr-1 (50 pg), NeuroD (500 pg), Notch-ICD (50 pg), or DN-Su(H) (300 pg), as indicated in the upper right hand corner. The antisense probes used are indicated in the lower left hand corner. The injected side (β-gal, light blue) is on the right, and embryos are shown as a dorsal view, anterior down. Red arrowhead marks ectopic expression (A, C) or the increased density of the lateral stripe (I, J). (K) Xmxi1 is activated by X-ngnr-1 and SoxD, but not by the neural inducer noggin. RT-PCR analysis of animal caps isolated from embryos injected with noggin (50 pg), X-ngnr-1 (25 pg), and SoxD (200 pg), as indicated. Histone H4 was used as a loading control. CC, control caps; CE, control embryos. (L, M) N-tubulin expression of stage 14 embryos injected with X-ngnr-1-MO (15 ng) and X-ngnr-1 (25 pg), as indicated in the upper right hand corner. (N) SoxD activation of Xmxi1 does not require X-ngnr-1. Animal caps were isolated from embryos injected with SoxD (200 pg), X-ngnr-1-MO (15 ng), X-ngnr-1 (25 pg), and control-MO (Co-MO) (15 ng), as indicated, cultured until sibiling embryos reached stage 14 and subject to real-time RT-PCR analysis. Expression levels were normalized to ornithine decarboxylase (ODC) and compared to the induction capacity of SoxD, which was set to 10. Note that X-ngnr-1 RT primers detect both endogenous and injected X-ngnr-1 RNA. |