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Fig. 3. Early zygotic expression of xMLK2 was detected by whole-mount in situ hybridization in the cement gland, pronephros, and brain. (A) xMLK2 mRNA was first detected at stage 22 in the cement gland (CG). (B) By stage 24, xMLK2 was noted most strongly in the peripheral of the cement gland. (C) Stage 25 embryos showed two concentric rings of expression around the cement gland. The eye anlage is outlined in gray. (D) In situ hybridization with the early cement gland marker, xCG (Sive et al., 1989), on stage 25 embryo showed staining around the cement gland. (E) Stage 30 embryo showing expression in the cement gland and in the pronephric anlage (PA). (F and G) Stage 32 and 36 embryos showing xMLK2 expression in the developing pronephros (P) and weakly in the pronephric duct (PD). (H) Stage 37, xMLK2 was expressed in the functional pronephros (P) and much more weakly in the pronephric duct. Panels are lateral views with anterior to left. |
