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Fig. 4
Effects of Six1 knockdown on early ectodermal TFs. a–j Expression of PPE (Eya1, Sox3), NC (FoxD3), neural plate (Sox3) markers and early ectodermal TFs in dorsal views of neural plate stage Xenopus embryos after injection of Six1 MO1 + MO2. Anterior is to the bottom. Control side is shown on the left and injected side on the right (as indicated by blue LacZ staining). Reductions (arrows) and increased or ectopic expression domains (asterisks) in the neural (green) and non-neural ectoderm (orange) compared with the control side (arrowheads) are indicated. Green lines highlight broadening of the neural plate and lateral displacement of NPB markers on the injected side (bright green) versus control side (dark green). Insets show alternative phenotypes. k Summary of regulatory interactions. Arrows indicate requirement of Six1 for expression of TFs (reduction after Six1 knockdown). Bars indicate requirement of Six1 for restriction of expression of TFs (increase after Six1 knockdown). Faint colors indicate less frequent phenotypes. See Additional file 1: Table S8 for numbers. |
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Fig. 5
Effects of Six1 overexpression on NPB markers and other ectodermal TFs. a–j Expression of PPE (Eya1, Sox3), NC (FoxD3), neural plate (Sox3) markers and early ectodermal TFs in dorsal views of neural plate stage Xenopus embryos after injection of Six1 mRNA. Anterior is to the bottom. Control side is shown on the left and injected side on the right (as indicated by red LacZ immunostaining). Arrowheads indicate expression domains on the control side. Reductions (arrows) and increased or ectopic expression domains (asterisks) in the neural (green) and non-neural ectoderm (orange) compared with the control side (arrowheads) are indicated. Insets show alternative phenotypes. k Summary of regulatory interactions. Arrows indicate ability of Six1 to promote expression of TFs. Bars indicate ability of Six1 to repress TFs. Faint colors indicate less frequent phenotypes. See Additional file 1: Table S9 for numbers. |
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Figure S5. Effects of Eya1 knockdown on NPB markers and other ectodermal TFs. A-J: Expression of PPE (Six1, Sox3), NC (FoxD3), neural plate (Sox3) markers and early ectodermal TFs in dorsal views of neural plate stage Xenopus embryos after injection of Eya1 MO1 + MO2. Anterior is to the bottom. Control side is shown on the left and injected side on the right (as indicated by blue LacZ staining). Arrowheads indicate expression domains on the control side. Reductions (arrows) and increased or ectopic expression domains (asterisks) in the neural (green) and non-neural ectoderm (orange) compared with the control side (arrowheads) are indicated. Green lines indicate broadening of the neural plate and lateral displacement of NPB markers on the injected side (bright green) versus control side (dark green). Insets show alternative phenotypes. K: Summary of regulatory interactions. Arrows indicate requirement of Eya1 for expression of TFs (reduction after Eya1 knockdown). Bars indicate requirement of Eya1 for restriction of expression of TFs (increase after Eya1 knockdown). Faint colors indicate less frequent phenotypes. See Additional file 1: Table S8 for numbers. (PDF 2013 kb) |
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Figure S6. Effects of Eya1 overexpression on NPB markers and other ectodermal TFs. A-J: Expression of PPE (Eya1, Sox3), NC (FoxD3), neural plate (Sox3) markers and early ectodermal TFs in dorsal views of neural plate stage Xenopus embryos after injection of Eya1 mRNA. Anterior is to the bottom. Control side is shown on the left and injected side on the right (as indicated by blue LacZ staining). Reductions (arrows) and increased or ectopic expression domains (asterisks) in the neural (green) and non-neural ectoderm (orange) compared with the control side (arrowheads) are indicated. Green lines indicate broadening of the neural plate and lateral displacement of NPB markers on the injected side (bright green) versus control side (dark green). Insets show alternative phenotypes. K: Summary of regulatory interactions. Arrows indicate ability of Six1 to promote expression of TFs. Bars indicate ability of Six1 to repress TFs. Faint colors indicate less frequent phenotypes. See Additional file 1: Table S9 for numbers. (PDF 1906 kb) |
