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Fig. 2. PRC2 regulates neural and neural crest development. MOs (20 ng) against Eed, Ezh2 or Suz12 were injected unilaterally into two-cell-stage embryos. The embryos were examined at neurula stages by ISH for neural and neural crest markers. Knockdown of either one of the PRC2 proteins reduced the expression of the late neural marker NRP1 and the neural crest genes Snail2 and Sox9, but did not drastically decrease the expression of the early neural marker Sox3 (open neural plate stages, first column from left; late neurula stages, second column from left) or the neural plate border genes AP2α and Msx1. The mesodermal marker MyoD was not inhibited either. Numbers indicate embryos showing the displayed patterns (no reduction for Sox3, AP2α, Msx1 and MyoD, and decreased expression for NRP1, Snail2 and Sox9) over the total number of embryos analyzed. |
