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msx1xenopus   

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Experiment details for msx1

Msx1 and Msx2 have shared essential functions in neural crest but may be dispensable in epidermis and axis formation in Xeno...

Msx1 and Msx2 have shared essential functions in neural crest but may be dispensable in epidermis and axis formation in Xenopus.

Gene Clone Species Stages Anatomy
msx1.L laevis NF stage 12.5 neuroectoderm , neural plate border
msx1.L laevis NF stage 14 neural crest , cranial neural crest , non-neural ectoderm
msx1.L laevis NF stage 16 neural crest , cranial neural crest , neural plate border

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  Fig. 1. Expression of Msx1 and Msx2 in Xenopus embryos. (A) Glyoxal RNA gel blots of 0.75 μg per lane of total RNA isolated from different embryonic stages probed with Msx1 and Msx2. Nieuwkoop-Faber stages are as indicated. Both transcripts appear first at early gastrula, level off at early neurula and are maintained at roughly constant amounts through mid tailbud stages. Ethidium bromide staining of the 18S ribosomal band is shown to control for equal RNA loading. (B) Whole mount in situ hybridization to late gastrula (St. 12.5) and early (St. 14) and mid-neurula (St. 16) embryos. The expression patterns of Msx1 and Msx2 are very similar. Expression of Slug is shown for comparison. Dorsal views, with anterior towards the top of the figure.