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Fig. 8. A low dose of Smad1 overexpression mimics Smurf1 knockdown. N-CAM staining is slightly reduced (A, B), while Xep (D, arrow) and Msx1 (G, arrowheads) are up-regulated at the periphery of the neural plate in embryos injected with 1 ng Smad1 mRNA. As a positive control, 6 ng Smad1 mRNA strongly induces Xep (E, arrow) and Msx1 (H, arrowheads) within the neural plate. Notice the wider neural plate in all Smad1-overexpressing embryos (B, D, E, G, H). All are anterior views at stage 14 (A, B) or 17 (C–H). |
