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Fig. 6. BMP-responsive genes are up-regulated by blocking Smurf1. (A–D) The zone of Msx1, a marker of the neural plate border, is wider in Smurf1 MO and Smurf1CA embryos (B, D, arrowheads), than in control embryos (A, C). (E–G) Expression pattern of the epidermal marker, Xep, encroaches into the placodal zone (F, arrow), but loses its neural domain (asterisk) in Smurf1CA embryos. Its normal pattern is rescued by wt Smurf1 (G). Pink color demarcates lineage tracing by β-gal. (H–L) Double staining of control, Smurf1 MO and Smurf1CA embryos with Xep (purple, arrows) and Sox2 (cyan) and rescue of the Xep pattern by wt Smurf1 (M) and Smad6 (N). All are anterior views. |
