| |
Fig. 6. Rostral shifting and expansion of somitomere and presomitic
mesoderm markers occurs in Rarβ2 MO-injected embryos. (A-F) Embryos
were injected unilaterally at the 2- or 4-cell stage with 26 ng Rarβ2.L MO+26 ng
Rarβ2.S MO. Injected side is indicated by magenta β-gal lineage tracer.
Neurula stage embryos shown in dorsal view with anterior on the left. Rarβ2
MOs rostrally shift somitomere markers Ripply2 (A) and Mespa/Thyl2 (B), and
thicken their boundaries of expression (Ripply2, 25/27 embryos; Mespa, 26/
31). The expression domains of presomitic mesoderm markers Tbx6 (C),
Msgn1 (D) and Fgf8 (E), and the Notch direct target Esr5 (F) are expanded
rostrally (red vertical lines) by Rarβ2 MOs (Tbx6, 26/28 embryos; Msgn1, 7/9;
Fgf8, 9/13; Esr5, 19/20). Broken red line indicates the midline. |
